Detection of antibodies towards Chlamydia species in hen serum


Avian chlamydiosis is a bacterial an infection attributable to Chlamydia micro organism. The at the moment accessible exams distinguish Chlamydia species primarily based on genetic materials. Researchers from Wageningen Bioveterinary Analysis (WBVR) developed a take a look at primarily based on antibodies current in animal blood that may decide which Chlamydia species contaminated the animal.

Chlamydia micro organism are gram-negative, intracellular micro organism. The bacterial genus at the moment consists of fourteen species, every with its personal most well-liked host. Quite a few these species cannot solely trigger illness of their most well-liked hosts, but in addition have zoonotic potential. The notorious zoonotic species Chlamydia psittaci has been present in a whole lot of fowl species, together with poultry. This bacterium could cause a extreme type of pneumonia in people, often known as parrot fever.

Differentiate between species
C. gallinacea and C. psittaci can infect birds, however each species differ in epidemiology and zoonotic potential. It’s subsequently necessary to have diagnostic instruments which allow detection and distinction between the micro organism species in poultry. The accessible exams are primarily based on molecular strategies (DNA). These exams solely present details about the present an infection standing of animals, and work if there are sufficient micro organism within the samples taken.

A crew of WBVR researchers led by Marloes Heijne and Fimme Jan van der Wal*, investigated whether or not it’s potential to develop a take a look at to detect antibodies towards Chlamydia species in poultry. Serology is appropriate for monitoring flocks, as antibodies stay current for a very long time after an an infection,” explains Heijne. At current, no differentiating take a look at is accessible for Chlamydia serology.”

New take a look at
To allow differentiating serology, a suspension array was designed, with peptides as antigens. These peptides are derived from identified immunoreactive Chlamydia proteins. For almost all of those peptides, species-specific seroreactivity in mammalian sera has been described within the literature. For the suspension array, the peptides had been immobilised on completely different colored spheres, a mixture of which was added to serum to seize the species-specific antibodies. Actually, a serological suspension array is just like an ELISA. Nonetheless, utilizing the array one can carry out a number of ELISA’s for one pattern concurrently, in a single response. For read-out of the assay we use devoted gear, a Luminex”, Heijne explains. With quite a few further measures, the take a look at was made appropriate to be used in a number of animal species. In our examine, we confirmed that quite a few peptides, which have been used within the literature as antigens for Chlamydia serology in mammals, additionally perform in detecting and differentiating antibodies towards the 2 for poultry necessary Chlamydia species.”

The suspension array can establish antibodies of 4 completely different Chlamydia species in mouse sera. The identical take a look at can also be in a position to distinguish between antibodies of C. psittaci and C. gallinacea in hen sera. As a result of the reactivity of hen sera was not the identical as that of mice, alerts from hen sera from the sector had been troublesome to interpret. Effectively-characterised hen sera for evaluating the seroreactivity of the peptides are scarce. However, outcomes with subject sera are according to revealed information on the incidence of C. gallinacea in Dutch laying hens. With this, we have now demonstrated the proof of idea of differentiating serology for antibodies towards Chlamydia in poultry utilizing peptides in a suspension array.”

* WBVR researcher Fimme Jan van der Wal is first creator of the scientific publication, revealed in Veterinary Analysis (2023)54:31

Supply: Wageningen College & Analysis


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